間眅埶AV

CORONAVIRUS TESTING KITS WILL BE DEVELOPED USING 間眅埶AV-INVENTED RNA IMAGING TECHNOLOGY

March 19, 2020
Facebook
Twitter
LinkedIn
Reddit
SMS
Email
Copy

間眅埶AV researchers will use their pioneering imaging technologycalled Mango, for its bright colour to develop coronavirus testing kits. Theyre among a small set of Canadian researchers who responded to the rapid funding opportunity recently  to 

間眅埶AV researchers Lena Dolgosheina, a post-doctoral fellow and Peter Unrau, a professor of molecular biology and biochemistry, developed Mango to sensitively detect RNA molecules, helping to improve viral screening for viruses such as the coronavirus while enabling basic discoveries into the functioning of cells.

The latest research, led by Unrau, involves using Mango to detect individual molecules of RNA within a living cell.

We are made of molecules so when something goes wrong within a cell it happens at the molecular level, says Unrau. We are using the Mango system as a catalyst, to allow us to not only extend fundamental research questions but also to detect pathogens like the coronavirus, faster and more efficiently. 

The Mango system consists of an RNA Mango aptamer that binds tightly and specifically to a fluorescent dye. The aptamer acts like a magnet targeting and binding those dye molecules. The dye becomes excitable when bound and glows brightly. RNA molecules modified to contain the aptamer 'magnet' now stand out from the other parts of the cell, which makes it much easier for researchers to see and study RNA molecules under a microscope.

Cell regulation takes place at the level of RNA, he says. For a long time, the focus has been on protein but it is RNA and not protein that regulates the vast majority of processes within a cell.

RNA Mango dyes are currently available from Applied Biological Materials (ABM) in Richmond, B.C. The coronavirus research made possible by CIHR funding will allow the team to develop an isothermal testing methodology, known as Mango NABSA (nucleic acid sequence-based amplification).

The Mango NABSA kits can be used to test for the coronavirus, which is a positive strand RNA virus. ABM is actively involved with this project as a partner and will supply the enzymes and buffers needed, which the 間眅埶AV team originally developed.

Mango technology is state of the art and the development of effective cures for cancer and other diseases demand better imaging methodologies to rapidly learn how cells work in detail, Unrau adds. 

The teams research is published in 

CLICK  IF YOU ARE INTERESTED IN DONATING TO 間眅埶AV'S CAMPAIGN SUPPORTING PETER UNRAU'S COVID RESEARCH