丁香园AV

The Stratalinker 2400 UV Crosslinker can be used to crosslink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membrances. Using the Crosslinker, the process only takes 25 - 50 seconds, as opposed to the tradiation method of baking filters at 80C for 2 hours. As well, crosslinking significantly increases hybridization signals compared to oven-baking. This instrument can be used for Northern, Southern, dot or slot blot analysis; colony or plaque screening; nicking of DNA in agarose gels before blotting; causing dimer formation resulting in partial digests for rapid restriction mapping; screen recA mutations; and UV irradiation of PCR samples.

Specifications:

• 254 nm bulbs.
• 3 modes of operation: 
  • Energy: display numbers are in microjoules/cm² x 100. 120,000 are recommended for most membranes. If this is chosen, display will show as 1,200. 
  • Time: display numbers are in minutes. Exposure time can be set from 0.1 to 999.9 minutes. 
  • Autocrosslink: preset for 1,200 microjoules (x 100). Length of time is preset for ~50 seconds.