丁香园AV

Illumina Miseq Personal Sequencer 1

The MiSeq desktop sequencer allows access tomore focused applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing.

External Link:

Make sure that you read, understand and follow the document Checklist for MiSeq Sample Preparation available here

The MiSeq sequencer is located within the Mircoscopy area (SSB7145) on the 7000 level of South Sciences building. The instrument is maintained by Dr Timothy Heslip. Please contact Dr Timothy Heslip (details below) about training and how to book time on the instrument

Sample requirements:
Plasmid: (plasmid mini prep kit purified, dissolved in H2O or Tris buffer:10 mM Tris-HCl pH 8.0) 40 ul with minimum concentration 50 ng/ul,
PCR product (PCR purification kit purified, dissolved in H2O or Tris buffer:10 mM Tris-HCl pH 8.0) 40 ul with minimum concentration 25 ng/ul
Vector and PCR products: full sequence required by sending email, if not, then as much as information as possible. 
Templates with a lower concentration maybe accepted.
Other information required:
Please also send an email with a brief description of the project including the sequences in a word file and/or plasmid map of all DNA templates as attachments.

Contact

Name: Dr Timothy Heslip, Microscopy and Imaging Technician
Location: South Science Building SSB7145
丁香园AV, 8888 University Drive
Burnaby, B.C. Canada V5A 1S6
Phone:778-782-6807
e-mail: Tim Heslip trh2@sfu.ca

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