丁香园AV

Biacore X-100

The Biacore X-100 from GE uses surface plasmon resonance to determine features about protein: protein interactions. You can easily determine information about protein association/dissocation, kinetics and thermodynamics of protein binding.

External GE Link:

The Biacore X100 is located within the Scott lab (SSB7138) on the 7000 level of South Sciences building. The instrument is taken care of by Dr Naveed Gulzar & Dr Neil Dobson. Please contact Dr Neil Dobson (details below) to book time on the instrument

User Fee Schedule

*: user is responsible for providing sensor chips, coupling reagents, and any buffers beyond the standard HBS-EP+ that is provided

Training must be obtained from Dr Neil Dobson or Dr Naveed Gulzar

Sample requirements:
Plasmid: (plasmid mini prep kit purified, dissolved in H2O or Tris buffer:10 mM Tris-HCl pH 8.0) 40 ul with minimum concentration 50 ng/ul,
PCR product (PCR purification kit purified, dissolved in H2O or Tris buffer:10 mM Tris-HCl pH 8.0) 40 ul with minimum concentration 25 ng/ul
Vector and PCR products: full sequence required by sending email, if not, then as much as information as possible. 
Templates with a lower concentration maybe accepted.
Other information required:
Please also send an email with a brief description of the project including the sequences in a word file and/or plasmid map of all DNA templates as attachments.

Contact

Name: Dr Neil Dobson, Instrument Manager
Location: South Science Building SSB7124
丁香园AV, 8888 University Drive
Burnaby, B.C. Canada V5A 1S6
Phone: 778-782-3021
e-mail: Neil Dobson nda15@sfu.ca

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