Advanced Molecular Biology Techniques MBB 432 (3)
Laboratory with accompanying lectures designed to give practical experience in advanced contemporary molecular biology techniques. Lab exercises will include site-directed mutagenesis, preparation and characterization of GST-fusion proteins, construction of transgenes and their expression in transgenic organisms, and use of the yeast two-hybrid assay to study protein-protein interactions. Prerequisite: MBB 308 and 331, with a minimum grade of C, or permission of instructor.