5. Ultra Violet Lamps inside Biosafety Cabinet
Does your biological safety cabinet (BSC) have an ultraviolet (UV) lamp in it? If so, it may not be as effective for sterilization/decontamination purposes as you need it to be. The sterilization/decontamination activity of UV lights is limited by a number of factors.
UV light loses its intensity over time. Also, anything that is left in the cabinet can create a shadow that prevents the UV light from decontaminating the affected area. In addition, the UV light must be cleaned regularly because dust can affect the ability of the UV light to decontaminate.
For these reasons and other safety concern (UV light not only kills microorganisms in the biosafety cabinet, but it also destroys living cells in your body), UV lamps are not recommended or necessary for decontamination in biosafety cabinets. Proper use and cleaning of biosafety cabinets (e.g. 10% bleach followed by 70% EtOH) negate any need for the use of UV lamps.
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